Scrt2^em1Zyliu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Scrt2^em1Zyliu
• Name: scratch family zinc finger 2; endonuclease-mediated mutation 1, Zhiyong Liu
• MGI ID: MGI:6368164
• Synonyms: Scrt2-tdTomato
• Gene: Scrt2 Location: Chr2:151923737-151937722 bp, + strand Genetic Position: Chr2, 74.83 cM
• Alliance: Scrt2^em1Zyliu page

Mutation origin

• Strain of Origin: C57BL/6 x DBA

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: Using CRISPR/Cas9 technology with sgRNA 5'-GCCTCGGCGGGCATCCCGCA-3', sequence for the P2A self-cleaving peptide sequence plus the tdTomato red fluorescent reporter gene was inserted immediately before the stop codon. (J:293995)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Scrt2 Mutation: 16 strains or lines available

References

• Original: J:293995 Li C, et al., Comprehensive transcriptome analysis of cochlear spiral ganglion neurons at multiple ages. Elife. 2020 Jan 8;9:e50491
• All: 2 reference(s)