Psmb11^em1.1Yout
Endonuclease-mediated Allele Detail

Summary

• Symbol: Psmb11^em1.1Yout
• Name: proteasome (prosome, macropain) subunit, beta type, 11; endonuclease-mediated mutation 1.1, Yousuke Takahama
• MGI ID: MGI:6368033
• Synonyms: beta5t-G49S-knock-in
• Gene: Psmb11 Location: Chr14:54862767-54867013 bp, + strand Genetic Position: Chr14, 27.87 cM
• Alliance: Psmb11^em1.1Yout page

Mutation origin

• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Hypomorph)
• Mutation: Single point mutation
• Mutation details: A G to A (c.145G>C) mutation was engineered to change glycine codon 49 (GGC) to a serine codon (AGC) (p.G49S) in a genomic sequence fragment in the targeting vector, which also contains A loxP site flanked neomycin resistance gene cassette inserted into an intron. The targeting vector was injected into ES cells together with two gRNAs and CRISPR/Cas9 nickase mRNA encoding plasmid. The neo cassette was removed through subsequent cre-mediated recombination. The mutation mimics a polymorphism found in the human population. (J:278028)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Psmb11 Mutation: 13 strains or lines available

References

• Original: J:278028 Ohigashi I, et al., A human PSMB11 variant affects thymoproteasome processing and CD8+ T cell production. JCI Insight. 2017 May 18;2(10)
• All: 1 reference(s)