Rnf144a^tm1Wclin
Targeted Allele Detail

Summary

• Symbol: Rnf144a^tm1Wclin
• Name: ring finger protein 144A; targeted mutation 1, Weei-Chin Lin
• MGI ID: MGI:6367987
• Gene: Rnf144a Location: Chr12:26356796-26465296 bp, - strand Genetic Position: Chr12, 9.69 cM, cytoband A3
• Alliance: Rnf144a^tm1Wclin page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267523
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette HTGR6005_A_5_G08 was inserted at position 26376992 of Chromosome 12 upstream of the critical exons 5 and 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 5 and 6 at position 26378094. The critical exons 5 and 6 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:267523)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnf144a Mutation: 23 strains or lines available

References

• Original: J:267523 Ho SR, et al., RNF144A sustains EGFR signaling to promote EGF-dependent cell proliferation. J Biol Chem. 2018 Oct 19;293(42):16307-16323
• All: 2 reference(s)