Prkag2^em1Blsg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Prkag2^em1Blsg
• Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit; endonuclease-mediated mutation 1, Bao-Liang Song
• MGI ID: MGI:6367663
• Synonyms: H530R-PRKAG2
• Gene: Prkag2 Location: Chr5:25067742-25305640 bp, - strand Genetic Position: Chr5, 11.93 cM
• Alliance: Prkag2^em1Blsg page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Humanized sequence, Hypomorph)
• Mutation: Single point mutation
• Mutation details: Using CRISPR/Cas9 technology with sgRNA 5'-CTCTTTGCAGGTCCATCGGC-3' and a 121-bp single-stranded oligodeoxynucleotide (ssODN) template, a c.1580A>G point mutation was generated to change histidine codon 527 to an arginine codon (p.His527Arg). This mutation mimics the p.His530Arg mutation found in some human familial Wolff-Parkinson-White (WPW) syndrome patients. (J:278059)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkag2 Mutation: 55 strains or lines available

References

• Original: J:278059 Xie C, et al., Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome. Cell Res. 2016 Oct;26(10):1099-1111
• All: 1 reference(s)