Gria4^tm1Ksak
Targeted Allele Detail

Summary

• Symbol: Gria4^tm1Ksak
• Name: glutamate receptor, ionotropic, AMPA4 (alpha 4); targeted mutation 1, Kenji Sakimura
• MGI ID: MGI:6363976
• Synonyms: Gria4^flox
• Gene: Gria4 Location: Chr9:4417896-4796234 bp, - strand Genetic Position: Chr9, 2.46 cM
• Alliance: Gria4^tm1Ksak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279639
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The loxP sequence and Neo cassette flanked by two frt sites were inserted into a site 331 bp upstream of exon 12. An internal ribosomal entry site (IRES) sequence and splice donor (SD) sequence from exon 8 of the mouse Hprt gene were inserted downstream of the Neo cassette for the poly-A trapping strategy. The other loxP site was introduced into a site 185 bp downstream of exon 12 to eliminate the putative transmembrane domain after Cre-mediated recombination. (J:279639)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gria4 Mutation: 71 strains or lines available

References

• Original: J:279639 Rubio ME, et al., The number and distribution of AMPA receptor channels containing fast kinetic GluA3 and GluA4 subunits at auditory nerve synapses depend on the target cells. Brain Struct Funct. 2017 Nov;222(8):3375-3393
• All: 1 reference(s)