Ncr1^em1Gtbz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ncr1^em1Gtbz
• Name: natural cytotoxicity triggering receptor 1; endonuclease-mediated mutation 1, Gabrielle T Belz
• MGI ID: MGI:6363567
• Synonyms: NCR^B6C14R
• Gene: Ncr1 Location: Chr7:4340723-4348163 bp, + strand Genetic Position: Chr7, 2.5 cM
• Alliance: Ncr1^em1Gtbz page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Hypomorph)
• Mutation: Single point mutation
• Mutation details: CRISPR/Cas9 technology was used with sgRNA 5'-TAGGGCTATGTCTGAGCCAG-3' and a donor oligonucleotide to generate a T>C mutation changing cysteine codon 14 (TGT) to an arginine codon (CGT) (p.C14R). This mutation has spontaneously arisen in some Ly5.1 (B6.SJL-Ptprca Pepcb/BoyJ) colonies. (J:278854)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncr1 Mutation: 36 strains or lines available

References

• Original: J:278854 Almeida FF, et al., A point mutation in the Ncr1 signal peptide impairs the development of innate lymphoid cell subsets. Oncoimmunology. 2018;7(10):e1475875
• All: 2 reference(s)