Ripk1^em1Xlin
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ripk1^em1Xlin
• Name: receptor (TNFRSF)-interacting serine-threonine kinase 1; endonuclease-mediated mutation 1, Xin Lin
• MGI ID: MGI:6363437
• Synonyms: Ripk1^K376R
• Gene: Ripk1 Location: Chr13:34186346-34221130 bp, + strand Genetic Position: Chr13, 14.01 cM
• Alliance: Ripk1^em1Xlin page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Single point mutation
• Mutation details: Using CRISPR/Cas9 technology with an sgRNA targeting the sequence around lysine codon 376 plus a donor oligonucleotide containing sequence for a an arginine codon at that position, an allele was created with an-A to-G substitution leading to a p.K376R substitution in the Intermediate Domain. (J:279494)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ripk1 Mutation: 32 strains or lines available

References

• Original: J:279494 Tang Y, et al., K63-linked ubiquitination regulates RIPK1 kinase activity to prevent cell death during embryogenesis and inflammation. Nat Commun. 2019 Sep 13;10(1):4157
• All: 1 reference(s)