Gorasp2^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gorasp2^{tm1d(EUCOMM)Hmgu}
• Name: golgi reassembly stacking protein 2; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6363353
• Synonyms: Gorasp2^-
• Gene: Gorasp2 Location: Chr2:70491520-70522069 bp, + strand Genetic Position: Chr2, 41.82 cM
• Alliance: Gorasp2^{tm1d(EUCOMM)Hmgu} page
• IMPC: Gorasp2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:274869
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 70509347 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:274869)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gorasp2 Mutation: 31 strains or lines available

References

• Original: J:274869 Chiritoiu M, et al., GRASP55 and UPR Control Interleukin-1beta Aggregation and Secretion. Dev Cell. 2019 Apr 8;49(1):145-155.e4
• All: 2 reference(s)