Gorasp2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gorasp2^{tm1c(EUCOMM)Hmgu}
• Name: golgi reassembly stacking protein 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6363352
• Synonyms: Gorsap2^fl
• Gene: Gorasp2 Location: Chr2:70491520-70522069 bp, + strand Genetic Position: Chr2, 41.82 cM
• Alliance: Gorasp2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Gorasp2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:274869
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 70509347 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:274869)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gorasp2 Mutation: 31 strains or lines available

References

• Original: J:274869 Chiritoiu M, et al., GRASP55 and UPR Control Interleukin-1beta Aggregation and Secretion. Dev Cell. 2019 Apr 8;49(1):145-155.e4
• All: 2 reference(s)