Crb1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Crb1^{tm1c(EUCOMM)Hmgu}
• Name: crumbs family member 1, photoreceptor morphogenesis associated; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6363347
• Synonyms: Crb1^fl, Crb1 Flox
• Gene: Crb1 Location: Chr1:139124794-139304838 bp, - strand Genetic Position: Chr1, 60.87 cM
• Alliance: Crb1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Crb1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:279263
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 139255640 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 139256561. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:279263)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crb1 Mutation: 91 strains or lines available

References

• Original: J:279263 Cho SH, et al., Targeted deletion of Crb1/Crb2 in the optic vesicle models key features of leber congenital amaurosis 8. Dev Biol. 2019 Sep 15;453(2):141-154
• All: 2 reference(s)