Tg(Dbh-COP4*L132C,-lacZ)B16-4Mik
Transgene Detail

Summary

• Symbol: Tg(Dbh-COP4*L132C,-lacZ)B16-4Mik
• Name: transgene insertion B16-4, M I Kotlikoff
• MGI ID: MGI:6361518
• Synonyms: B16-Dbh^BAC-CaCh2_IRES_lacZ
• Transgene: Tg(Dbh-COP4*L132C,-lacZ)B16-4Mik Location: unknown
• Alliance: Tg(Dbh-COP4*L132C,-lacZ)B16-4Mik page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL/J)F2

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The ~188.5 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-354N13 was obtained; containing the entire dopamine beta-hydroxylase locus (Dbh), as well as ~106 kbp centromeric flanking sequences (including the complete Adamtsl2 locus) and ~65 kbp telomeric flanking sequences (including the complete Sardh and Sardhos loci). Using homologous recombination/BAC recombineering, a 5640 bp CatCh-IRES-lacZ-pA construct was inserted into the ATG start site of the BAC Dbh gene. No other loci on the BAC were altered. The 11.6 kbp BAC vector (pBA Ce3.6) contained a chloramphenicol selection cassette. Founder line B16-4 was identified with high lacZ expression in the expected cells. The calcium translocating channelrhodopsin cDNA sequence used here encodes the first 309 amino acids of green alga Chlamydomonas reinhardtii channelrhodopsin-2 that has been modified with an L132C amino acid substitution (CTC-to-TGC) designed to cause enhanced Ca2+ permeability. (J:314125)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:314125 Lee FK, et al., Genetically engineered mice for combinatorial cardiovascular optobiology. Elife. 2021 Oct 29;10:e67858
• All: 1 reference(s)