Eef1a1^tm1(tTA)Kuw
Targeted Allele Detail

Summary

• Symbol: Eef1a1^tm1(tTA)Kuw
• Name: eukaryotic translation elongation factor 1 alpha 1; targeted mutation 1, Kay-Uwe Wagner
• MGI ID: MGI:6360534
• Synonyms: EF1-LSL-tTA
• Gene: Eef1a1 Location: Chr9:78385735-78389006 bp, - strand Genetic Position: Chr9, 43.65 cM, cytoband E1
• Alliance: Eef1a1^tm1(tTA)Kuw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:292860
• Parent Cell Line: S1B6 (ES Cell)
• Strain of Origin: 129S1/SvImJ or C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Inducible, Null/knockout)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A conventional homologous recombination approach in mouse embryonic stem (ES) cells was used for the targeted insertion of the tetracycline-controlled transactivator (tTA) into intron 1 of the endogenous eukaryotic translation elongation factor 1 alpha 1 (Eef1a1) locus. The tTA is preceded by a splice acceptor site and a floxed transcriptional Stop sequence (i.e., promoterless neo with three polyA signals). (J:292860)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Eef1a1 Mutation: 60 strains or lines available

References

• Original: J:292860 Sakamoto K, et al., Efficient tissue-type specific expression of target genes in a tetracycline-controlled manner from the ubiquitously active Eef1a1 locus. Sci Rep. 2020 Jan 14;10(1):207
• All: 2 reference(s)