Rab28^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rab28^{tm1c(EUCOMM)Hmgu}
• Name: RAB28, member RAS oncogene family; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6360452
• Synonyms: Rab28^fl
• Gene: Rab28 Location: Chr5:41782319-41865500 bp, - strand Genetic Position: Chr5, 22.58 cM, cytoband B2
• Alliance: Rab28^{tm1c(EUCOMM)Hmgu} page
• IMPC: Rab28 gene page

Mutation origin

• Mutant Cell Line: HEPD0677_7_A05
• Germline Transmission: Earliest citation of germline transmission: J:267522
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 41855482 of Chromosome 5 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 41856303. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele is created by flp recombinase expression in mice carrying this allele. (J:267522)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab28 Mutation: 16 strains or lines available

References

• Original: J:267522 Ying G, et al., The small GTPase RAB28 is required for phagocytosis of cone outer segments by the murine retinal pigmented epithelium. J Biol Chem. 2018 Nov 9;293(45):17546-17558
• All: 1 reference(s)