Axin2^tm1.1Rva
Targeted Allele Detail

Summary

• Symbol: Axin2^tm1.1Rva
• Name: axin 2; targeted mutation 1.1, Renee van Amerongen
• MGI ID: MGI:6360044
• Synonyms: Axin2 P2A-rtTA3-T2A-3xNLS-sGFP2
• Gene: Axin2 Location: Chr11:108811175-108841609 bp, + strand Genetic Position: Chr11, 71.83 cM
• Alliance: Axin2^tm1.1Rva page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:297268
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Reporter, Transactivator)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct contains a P2A, a reverse tetracycline transactivator (rtTA3), a T2A, an SGFP2 (strongly enhanced green fluorescent protein) with a 3XNLS (nuclear localization signal) followed by an FRT-flanked PGK neo cassette (in reverse orientation) inserted at the stop codon (TGA) in exon 11. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:297268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Axin2 Mutation: 44 strains or lines available

References

• Original: J:297268 van de Moosdijk AAA, et al., A novel Axin2 knock-in mouse model for visualization and lineage tracing of WNT/CTNNB1 responsive cells. Genesis. 2020;58(9):e23387
• All: 3 reference(s)