Lipa^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Lipa^{tm1b(EUCOMM)Hmgu}
• Name: lysosomal acid lipase A; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6359482
• Gene: Lipa Location: Chr19:34469718-34504874 bp, - strand Genetic Position: Chr19, 29.7 cM
• Alliance: Lipa^{tm1b(EUCOMM)Hmgu} page
• IMPC: Lipa gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277763
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34488106 of Chromosome 19 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream exon 4 at position 34489566. Exon 4 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:277763)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lipa Mutation: 20 strains or lines available

References

• Original: J:277763 Pajed L, et al., Hepatocyte-specific deletion of lysosomal acid lipase leads to cholesteryl ester but not triglyceride or retinyl ester accumulation. J Biol Chem. 2019 Jun 7;294(23):9118-9133
• All: 1 reference(s)