Asxl1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Asxl1^{tm1c(EUCOMM)Wtsi}
• Name: ASXL transcriptional regulator 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6358618
• Synonyms: ASXL1^f
• Gene: Asxl1 Location: Chr2:153187750-153245927 bp, + strand Genetic Position: Chr2, 75.41 cM
• Alliance: Asxl1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Asxl1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267079
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt2 cassette was inserted at position 153198539 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 153199386. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:267079)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Asxl1 Mutation: 116 strains or lines available

References

• Original: J:267079 Rohatgi N, et al., ASXL1 impairs osteoclast formation by epigenetic regulation of NFATc1. Blood Adv. 2018 Oct 9;2(19):2467-2477
• All: 1 reference(s)