Bcar1^tm1Ike
Targeted Allele Detail

Summary

• Symbol: Bcar1^tm1Ike
• Name: breast cancer anti-estrogen resistance 1; targeted mutation 1, Kazuhiro Ikenaka
• MGI ID: MGI:6358378
• Synonyms: p130Cas-STOP-tetO
• Gene: Bcar1 Location: Chr8:112437106-112470441 bp, - strand Genetic Position: Chr8, 57.98 cM, cytoband D3
• Alliance: Bcar1^tm1Ike page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278505
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: A Neo STOP-tetO cassette was inserted just upstream of the Bcar1 translation initiation site. The cassette contains the following elements: a loxP site, an FRT site, a neomycin resistance gene cassette, a transcription and translation STOP cassette, an FRT site, a tetO cassette (the tetracycline operon site and a CMV minimal promoter) and a loxP site. In the absence of tetracycline transactivator (tTA) activity this allele is a null allele. The gene can be activated by expressing tTA (by crossing with a tTA transgenic mouse) in the absence of tetracycline family antibiotics. (J:278505)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bcar1 Mutation: 27 strains or lines available

References

• Original: J:278505 Shimizu T, et al., Mechanical regulation of oligodendrocyte morphology and maturation by the mechanosensor p130Cas. J Neurochem. 2019 Jul;150(2):158-172
• All: 1 reference(s)