Lgals3^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Lgals3^{tm1d(EUCOMM)Wtsi}
• Name: lectin, galactose binding, soluble 3; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6358295
• Gene: Lgals3 Location: Chr14:47611317-47623624 bp, + strand Genetic Position: Chr14, 24.6 cM, cytoband C1
• Alliance: Lgals3^{tm1d(EUCOMM)Wtsi} page
• IMPC: Lgals3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278316
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 47618595 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 47619361. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:278316)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgals3 Mutation: 29 strains or lines available

References

• Original: J:278316 Maupin KA, et al., Enhanced cortical bone expansion in Lgals3-deficient mice during aging. Bone Res. 2018;6:7
• All: 1 reference(s)