Lgr4^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Lgr4^{tm1c(EUCOMM)Hmgu}
• Name: leucine-rich repeat-containing G protein-coupled receptor 4; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6358096
• Synonyms: Lgr4^fl
• Gene: Lgr4 Location: Chr2:109747992-109844602 bp, + strand Genetic Position: Chr2, 56.65 cM
• Alliance: Lgr4^{tm1c(EUCOMM)Hmgu} page
• IMPC: Lgr4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:278480
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 109837067 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 109838661. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:278480)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr4 Mutation: 92 strains or lines available

References

• Original: J:278480 Li Z, et al., LGR4 protects hepatocytes from injury in mouse. Am J Physiol Gastrointest Liver Physiol. 2019 Jan 1;316(1):G123-G131
• All: 2 reference(s)