Bcas1^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Bcas1^{tm1d(EUCOMM)Wtsi}
• Name: brain enriched myelin associated protein 1; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6358056
• Gene: Bcas1 Location: Chr2:170188911-170269765 bp, - strand Genetic Position: Chr2, 91.41 cM
• Alliance: Bcas1^{tm1d(EUCOMM)Wtsi} page
• IMPC: Bcas1 gene page

Mutation origin

• Mutant Cell Line: EPD0336_1_H04
• Germline Transmission: Earliest citation of germline transmission: J:253194
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 170247962 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 170249252. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:253194)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bcas1 Mutation: 40 strains or lines available

References

• Original: J:253194 Fard MK, et al., BCAS1 expression defines a population of early myelinating oligodendrocytes in multiple sclerosis lesions. Sci Transl Med. 2017 Dec 6;9(419)
• All: 1 reference(s)