Gt(ROSA)26Sor^{em1(CAG-MIA2*P521A)Bcgen}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-MIA2*P521A)Bcgen}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Biocytogen LLC
• MGI ID: MGI:6358041
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-MIA2*P521A)Bcgen} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A vector containing the following elements was targeted to the host locus using CRISPR/Cas9 technology: The CAG promoter, a loxP site flnaked STOP cassette, three copies of the FLAG tag, the cDNA for the coding sequence of the human MIA2 gene containing the P521A point mutation, WPRE-probe, and poly-A signal. This creates a conditional-ready allele that will express the mutant human gene only after cre-mediated excision of the STOP cassette. (J:265909)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:265909 Zhang F, et al., cTAGE5/MEA6 plays a critical role in neuronal cellular components trafficking and brain development. Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):E9449-E9458
• All: 3 reference(s)