Mecr^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mecr^{tm1d(EUCOMM)Wtsi}
• Name: mitochondrial trans-2-enoyl-CoA reductase; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6357882
• Synonyms: Mecr^tm1d
• Gene: Mecr Location: Chr4:131570781-131595097 bp, + strand Genetic Position: Chr4, 64.52 cM
• Alliance: Mecr^{tm1d(EUCOMM)Wtsi} page
• IMPC: Mecr gene page

Mutation origin

• Mutant Cell Line: EPD0039_5_G01
• Germline Transmission: Earliest citation of germline transmission: J:242847
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 131580788 of Chromosome 4 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 2 at position 131581364. Exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 2. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. Western blot analysis confirmed the absence of protein expression in homozygous mutant embryos. No lipoylated proteins could be detected in E9.5 homozygous embryos, consistent with complete loss of Mecr function in this line. (J:242847)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mecr Mutation: 17 strains or lines available

References

• Original: J:242847 Nair RR, et al., Genetic modifications of Mecr reveal a role for mitochondrial 2-enoyl-CoA/ACP reductase in placental development in mice. Hum Mol Genet. 2017 Jun 01;26(11):2104-2117
• All: 1 reference(s)