Prkdc^tm4.1Shzh
Targeted Allele Detail

Summary

• Symbol: Prkdc^tm4.1Shzh
• Name: protein kinase, DNA activated, catalytic polypeptide; targeted mutation 4.1, Shan Zha
• MGI ID: MGI:6357218
• Synonyms: DNA-PKcs^PQR
• Gene: Prkdc Location: Chr16:15455730-15660099 bp, + strand Genetic Position: Chr16, 10.09 cM, cytoband B1
• Alliance: Prkdc^tm4.1Shzh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276977
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Five serine residues in the S2053 cluster in exon 46 (S2026, S2038, S2050, S2052, S2053) were replaced with alanines and an FRT flanked neo cassette was inserted in intron 45 via homologous recombination. Flp mediated recombination removed the neo cassette. These changes prevent phosphorylation at these residues in the mutant protein. (J:276977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkdc Mutation: 400 strains or lines available

References

• Original: J:276977 Jiang W, et al., Phosphorylation at S2053 in Murine (S2056 in Human) DNA-PKcs Is Dispensable for Lymphocyte Development and Class Switch Recombination. J Immunol. 2019 Jul 1;203(1):178-187
• All: 2 reference(s)