Prkdc^tm3.1Shzh
Targeted Allele Detail

Summary

• Symbol: Prkdc^tm3.1Shzh
• Name: protein kinase, DNA activated, catalytic polypeptide; targeted mutation 3.1, Shan Zha
• MGI ID: MGI:6357217
• Synonyms: DNA-PKcs^KDSD
• Gene: Prkdc Location: Chr16:15455730-15660099 bp, + strand Genetic Position: Chr16, 10.09 cM, cytoband B1

Mutation origin

• Germline Transmission: Unknown
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: The conserved aspartic acid at position 3922 was mutated to an alanine (p.D3922A) and a floxed neo cassette was inserted ~600 bp upstream via homologous recombination. Cre mediated recombination removed the neo cassette. In a second targeting event the conserved serine at position 2053 was mutated to an aspartic acid (p.S2053D) and an FRT flanked neo cassette was inserted in the upstream intron via homologous recombination. Flp mediated recombination removed the neo cassette. (J:220823)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkdc Mutation: 400 strains or lines available

References

• Original: J:220823 Jiang W, et al., Differential Phosphorylation of DNA-PKcs Regulates the Interplay between End-Processing and End-Ligation during Nonhomologous End-Joining. Mol Cell. 2015 Apr 2;58(1):172-85
• All: 1 reference(s)