Prkdc^tm1.1Shzh
Targeted Allele Detail

Summary

• Symbol: Prkdc^tm1.1Shzh
• Name: protein kinase, DNA activated, catalytic polypeptide; targeted mutation 1.1, Shan Zha
• MGI ID: MGI:6357215
• Synonyms: DNA-PKcs^KD
• Gene: Prkdc Location: Chr16:15455730-15660099 bp, + strand Genetic Position: Chr16, 10.09 cM, cytoband B1
• Alliance: Prkdc^tm1.1Shzh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220823
• Parent Cell Line: CSL3 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Single point mutation
• Mutation details: The conserved aspartic acid at position 3922 was mutated to an alanine (p.D3922A) and a floxed neo cassette was inserted ~600 bp upstream via homologous recombination. Cre mediated recombination removed the neo cassette. This mutation expresses a catalytically inactive mutant protein. (J:220823)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkdc Mutation: 400 strains or lines available

References

• Original: J:220823 Jiang W, et al., Differential Phosphorylation of DNA-PKcs Regulates the Interplay between End-Processing and End-Ligation during Nonhomologous End-Joining. Mol Cell. 2015 Apr 2;58(1):172-85
• All: 1 reference(s)