Ripk1^tm2.1Vmd
Targeted Allele Detail

Summary

• Symbol: Ripk1^tm2.1Vmd
• Name: receptor (TNFRSF)-interacting serine-threonine kinase 1; targeted mutation 2.1, Vishva M Dixit
• MGI ID: MGI:6356681
• Synonyms: Ripk1^RHIM
• Gene: Ripk1 Location: Chr13:34186346-34221130 bp, + strand Genetic Position: Chr13, 14.01 cM
• Alliance: Ripk1^tm2.1Vmd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242990
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: Sequence 5'-ATTCAGATTGGA-3', encoding RIP homotypic interaction motif (RHIM) residues IQIG, was replaced with 5'-GCTGCGGCTGCA-3', coding for AAAA. An FRT site flanked neomycin selection cassette inserted into intron 9 at position chr13:34,029,090 (GRCm38/mm10 assembly) was deleted from the targeted embryonic stem cells before microinjection. (J:242990)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ripk1 Mutation: 32 strains or lines available

References

• Original: J:242990 Newton K, et al., RIPK1 inhibits ZBP1-driven necroptosis during development. Nature. 2016 Dec 01;540(7631):129-133
• All: 3 reference(s)