Mmut^tm2.1Mrb
Targeted Allele Detail

Summary

• Symbol: Mmut^tm2.1Mrb
• Name: methylmalonyl-Coenzyme A mutase; targeted mutation 2.1, Matthias R Baumgartner
• MGI ID: MGI:6343343
• Synonyms: Mmut^tm1.1Pg, Mut^flox, Mut^tm1Bam
• Gene: Mmut Location: Chr17:41245576-41272879 bp, + strand Genetic Position: Chr17, 19.55 cM, cytoband C-D
• Alliance: Mmut^tm2.1Mrb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277356
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A conditional ready (floxed) allele was created from the KO first allele chimeras generated using a EUCOMM vector from IKMC project 47381. The cassette for the KO first allele is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon. The critical exon 3 is thus flanked by loxP sites. This conditional ready allele was created by removing the FRT-flanked lacZ sequence , loxP site, neomycin cassette under the control of the human beta-actin promoter and SV40 polyA via flp-mediated recombination, leaving exon 3 flanked by loxP sites. For vector details, see Mmut^{tm1a(EUCOMM)Wtsi}. (J:277356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mmut Mutation: 32 strains or lines available

References

• Original: J:277356 Remacle N, et al., New in vitro model derived from brain-specific Mut-/- mice confirms cerebral ammonium accumulation in methylmalonic aciduria. Mol Genet Metab. 2018 Aug;124(4):266-277
• All: 2 reference(s)