Col1a1^{tm23(CAG-Tol,-cre)Nki}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm23(CAG-Tol,-cre)Nki}
• Name: collagen, type I, alpha 1; targeted mutation 23, The Netherlands Cancer Institute
• MGI ID: MGI:6342020
• Synonyms: Col1a1^{tm23(Tol-CAG-cre)Nki}, Col1a1^{tm23(Tol-CAG-Kaede*,-Katushka*,-AG*,-BFP*,-mKO2,-eGFP*,-cre,-luc2)Nki}
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm23(CAG-Tol,-cre)Nki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:293819
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: RMCE retargeted ColaA1^tm22Nki, to replace the F3/frt flanked PGK-Puro casette in the 3' UTR of Cola1a by a "RMCE" plasmid encoding F3-CAG-Tol-frt, by cotransfecting a flpe-expressing plasmid to facilitate recombination into the 3' UTR of the Col1a1 locus. The transgene is a F3/FRT flanked cassette containing the CAG promoter, 8 scrambled reporter proteins, an E7 epitope and a HA tag. The 8 reporters are Kaede, Katushka, Azami Green, tagBFP, mKusabira-Orange2, eGFP, Cre recombinase and Luciferase2. All reporters are scrambled by dividing them in 3 parts, the cassette first holds the C-terminal regions of all reporters followed by all break regions and lastly all N-terminal regions. All epitopes of the 8 reporters should be expressed. (J:293819)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:293819 Bresser K, et al., A mouse model that is immunologically tolerant to reporter and modifier proteins. Commun Biol. 2020 May 29;3(1):273
• All: 1 reference(s)