H2-Eb2^{b-em1(IMPC)Wtsi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: H2-Eb2^{b-em1(IMPC)Wtsi}
• Name: histocompatibility 2, class II antigen E beta2; endonuclease-mediated mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6336054
• Synonyms: H2-Eb2^{em1(IMPC)Wtsi}
• Gene: H2-Eb2 Location: Chr17:34544639-34560386 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: H2-Eb2^{b-em1(IMPC)Wtsi} page
• IMPC: H2-Eb2 gene page

Mutation origin

• Strain of Origin: C57BL/6N
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at Wellcome Sanger Institute using Cas9 and guides with spacer sequences ATCGTCCCAAGGTCTGTCGA, CATCCTCACCACCTGAAGCA, GGGCTATGTATAAACCTTAT, and GTGTGATTAGAGTGTGCAGC that targeted exon(s) ENSMUSE00000411205.6 ENSMUSE00000608469.2. This resulted in deletion(s) of 1777 bp (location: Chr17:34552096-34553872; GRCm39). This description was generated automatically. Additional molecular information including FASTA sequence support for this allele can be found here: (IMPC Gene page) and viewed in a genome context here: (IMPC Genome Browser). (J:384794)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H2-Eb2 Mutation: 20 strains or lines available

References

• Original: J:237616 MGI and IMPC, MGI Curation of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). MGI Direct Data Submission. 2017-8
• All: 2 reference(s)