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Transgene Detail
Symbol: Tg(Cdh5-RHO/Adrb2,-lacZ)B27-3Mik
Name: transgene insertion B27-3, Michael I Kotlikoff
MGI ID: MGI:6324935
Transgene: Tg(Cdh5-RHO/Adrb2,-lacZ)B27-3Mik  Location: unknown  
Strain of Origin:  FVB/N X B6(Cg)-Tyrc-2J/J
Transgene Type:    Transgenic (Inserted expressed sequence, Reporter)
Mutation:    Insertion
Mutation detailsCdh5-Optoβ2AR-IRES-lacZ transgenic mice (also called Cdh5-Optoβ2AR-IRES-lacZ or Cdh5-Opto-β2AR-IRES-lacZ) were designed in the laboratory of Dr. Michael I. Kotlikoff (Cornell University) as part of Cornell/National Heart Lung Blood Resource for Optogenetic Mouse Signaling (CHROMus). The ~200 kbp C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-453P1 was obtained; containing the entire cadherin 5 locus (Cdh5) as well as ~124 kbp of 5' flanking sequences (including the complete Gm32728, Gm39236 and Gm8748 predicted gene loci) and ~33 kbp of 3' flanking sequences (including a 5' portion of the Bean1 locus). Using homologous recombination/BAC recombineering, a 5990 bp Optoβ2AR-IRES-lacZ-pA construct (Optoβ2AR, internal ribosome entry site, β-galactosidase and an SV40 polyadenylation signal) was inserted into the ATG start site of the BAC Cdh5 gene (replacing the initiation codon of Cdh5 in exon 2). (J:101977)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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