Mir211^tm1.1Dic
Targeted Allele Detail

Summary

• Symbol: Mir211^tm1.1Dic
• Name: microRNA 211; targeted mutation 1.1, Di Chen
• MGI ID: MGI:6324573
• Synonyms: miR-211^flox
• Gene: Mir211 Location: Chr7:63855554-63855659 bp, + strand Genetic Position: Chr7, 34.62 cM
• Alliance: Mir211^tm1.1Dic page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276908
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: IRES-lacZ reporter cassette as wells as a loxP site were inserted 171 bp upstream miR-204 stem loop and a loxP site plus a neomycin selection cassette were placed 365 bp downstream miRNA stem loops by homologous recombination in ES cells. The chimeric mice where crossed to flippase-expressing transgenic mice to remove Fret or F3 (a mutant of Frt) flanked lacZ and Neo cassettes, leaving two loxP sites immidiately flanking the miRNA stem-loops. (J:276908)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir211 Mutation: 2 strains or lines available

References

• Original: J:276908 Huang J, et al., The microRNAs miR-204 and miR-211 maintain joint homeostasis and protect against osteoarthritis progression. Nat Commun. 2019 Jun 28;10(1):2876
• All: 2 reference(s)