Cetn3tm1c(EUCOMM)Wtsi
Targeted Allele Detail
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Symbol: |
Cetn3tm1c(EUCOMM)Wtsi |
Name: |
centrin 3; targeted mutation 1c, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:6324196 |
Synonyms: |
Cetnfl |
Gene: |
Cetn3 Location: Chr13:81931196-81945274 bp, + strand Genetic Position: Chr13, 43.68 cM, cytoband C3
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Alliance: |
Cetn3tm1c(EUCOMM)Wtsi page
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IMPC: |
Cetn3 gene page |
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Mutant Cell Lines: |
EPD0630_2_B09, EPD0630_2_E12 |
Germline Transmission: |
Earliest citation of germline transmission:
J:275959
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 81934502 of Chromosome 13 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 81935301. Exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:275959)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Cetn3 Mutation: |
13 strains or lines available
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Cell lines EPD0630_2_E12 and_ EPD0630_2_B09 were successfully used to make chimeric mice. Germline transmission was accomplished for both lines. J:275959
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Original: |
J:275959 Ying G, et al., Deletion of both centrin 2 (CETN2) and CETN3 destabilizes the distal connecting cilium of mouse photoreceptors. J Biol Chem. 2019 Mar 15;294(11):3957-3973 |
All: |
1 reference(s) |
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