Ube2d1^{tm3e(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ube2d1^{tm3e(EUCOMM)Wtsi}
• Name: ubiquitin-conjugating enzyme E2D 1; targeted mutation 3e, Wellcome Trust Sanger Institute
• MGI ID: MGI:6324191
• Gene: Ube2d1 Location: Chr10:71090810-71121092 bp, - strand Genetic Position: Chr10, 36.85 cM
• Alliance: Ube2d1^{tm3e(EUCOMM)Wtsi} page
• IMPC: Ube2d1 gene page

Mutation origin

• Mutant Cell Line: MEPD1008_4_C01
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 71096138 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ube2d1 Mutation: 22 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)