Tmem132a^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tmem132a^{tm1c(KOMP)Wtsi}
• Name: transmembrane protein 132A; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6323660
• Synonyms: Tmem132a^flox
• Gene: Tmem132a Location: Chr19:10835186-10847304 bp, - strand Genetic Position: Chr19, 7.26 cM
• Alliance: Tmem132a^{tm1c(KOMP)Wtsi} page
• IMPC: Tmem132a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104881
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 10843589 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 10844993. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tmem132a Mutation: 49 strains or lines available

References

• Original: J:104881 The RIKEN BioResource Center, Information obtained from The RIKEN BioResource Center. Unpublished. 2006-2013
• All: 3 reference(s)