Gt(ROSA)26Sor^{tm1.5(CAG-EGFP)Pjen}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.5(CAG-EGFP)Pjen}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.5, Patricia Jensen
• MGI ID: MGI:6322971
• Synonyms: RC::RG
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.5(CAG-EGFP)Pjen} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226320
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a rox-flanked His3-SV40 transcriptional STOP cassette, a frt-flanked His3-SV40 transcriptional STOP cassette, a loxP site, a cDNA sequence encoding tdTomato fluorescent protein, a His3-SV40 transcriptional STOP cassette, a bovine growth hormone polyA signal, a second loxP site, a cDNA sequence encoding enhanced green fluorescent protein (eGFP), a woodchuck hepatitis virus post-transcriptional regulatory element, a bovine growth hormone polyA signal, and an attB/attP-flanked PGK-frt-Neo-polyA cassette. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. Transient transfection with a PhiC31-expressing plasmid removes the attB/attP-flanked PGK-frt-Neo-polyA cassette and replaces it with the recombined attB/attP site (attL). Cre- and Flp-mediated recombination removed the tdTomato reporter. (J:226320)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:226320 Plummer NW, et al., Expanding the power of recombinase-based labeling to uncover cellular diversity. Development. 2015 Dec 15;142(24):4385-93
• All: 4 reference(s)