Serf2^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Serf2^{tm1d(EUCOMM)Hmgu}
• Name: small EDRK-rich factor 2; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6317359
• Gene: Serf2 Location: Chr2:121279679-121283901 bp, + strand Genetic Position: Chr2, 60.39 cM
• Alliance: Serf2^{tm1d(EUCOMM)Hmgu} page
• IMPC: Serf2 gene page

Mutation origin

• Mutant Cell Line: HAR0000_1_H02
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 121280074 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 121280814. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Serf2 Mutation: 16 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 2 reference(s)