Thra^em6Ffla
Endonuclease-mediated Allele Detail

Summary

• Symbol: Thra^em6Ffla
• Name: thyroid hormone receptor alpha; endonuclease-mediated mutation 6, Frederic Flamant
• MGI ID: MGI:6317007
• Synonyms: Thra^Slox, TRalpha^S3
• Gene: Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
• Alliance: Thra^em6Ffla page

Mutation origin

• Strain of Origin: 129

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutation: Undefined
• Mutation details: CRISPR-targeting in embryos carrying Thra^tm1Ffla with a 5' floxed neo cassette, a cDNA encoding the open reading frame with a nucleotide substitution that results in an amino acid substitution of arginine for leucine at position 400 (L400R), and a 3' IRES-Tau-lacZ cassette resulted in a frameshift mutation after position 393 and a gain of stop codon at position 401. (J:268664)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thra Mutation: 39 strains or lines available

References

• Original: J:268664 Markossian S, et al., CRISPR/Cas9 Editing of the Mouse Thra Gene Produces Models with Variable Resistance to Thyroid Hormone. Thyroid. 2018 Jan;28(1):139-150
• All: 1 reference(s)