Col1a1^{tm18(CAG-Gata3,-luc2)Nki}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm18(CAG-Gata3,-luc2)Nki}
• Name: collagen, type I, alpha 1; targeted mutation 17, The Netherlands Cancer Institute
• MGI ID: MGI:6316337
• Synonyms: Col1a1^{invCAG-mGATA3-TR2-IRES-Luc}
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm18(CAG-Gata3,-luc2)Nki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276501
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: RMCE introduced into the 3' UTR an FRT-flanked cassette containing an inverted lox71/lox66-flanked CAG promoter, the mouse Gata3 sequence lacking the second GATA-type zinc-finger and the C-terminus (1-307), IRES sequence and firefly luciferase reporter gene (fLuc2). (J:276501)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

Notes

Mutation created in ES cells containing Cdh1^tm1Jjon and Tg(Wap-cre)51Nki, which were subsequently bred out of the line.

References

• Original: J:276501 Cornelissen LM, et al., GATA3 Truncating Mutations Promote Cistromic Re-Programming In Vitro, but Not Mammary Tumor Formation in Mice. J Mammary Gland Biol Neoplasia. 2019 Jun 19
• All: 1 reference(s)