Zfp365^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zfp365^{tm1b(KOMP)Wtsi}
• Name: zinc finger protein 365; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6316333
• Gene: Zfp365 Location: Chr10:67721935-67748492 bp, - strand Genetic Position: Chr10, 35.04 cM
• Alliance: Zfp365^{tm1b(KOMP)Wtsi} page
• IMPC: Zfp365 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:104881
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 67744796 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 67746210. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:104881)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp365 Mutation: 22 strains or lines available

References

• Original: J:104881 The RIKEN BioResource Center, Information obtained from The RIKEN BioResource Center. Unpublished. 2006-2013
• All: 2 reference(s)