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Endonuclease-mediated Allele Detail
Symbol: Plcg2em1Msasn
Name: phospholipase C, gamma 2; endonuclease-mediated mutation 1, Michael Sasner
MGI ID: MGI:6316314
Synonyms: Plcg2P522R, Plcg2R522
Gene: Plcg2  Location: Chr8:118225030-118361881 bp, + strand  Genetic Position: Chr8, 64.26 cM
Strain of Origin:  B6(SJL)-Apoetm1.1(APOE*4)Adiuj/J
Allele Type:    Endonuclease-mediated (Humanized sequence)
Mutation:    Single point mutation
Mutation detailsA C-to-G mutation was engineered in proline codon 522 (CCC) to change it to an arginine codon (CGC) (c.1565C>G, p.P522R) with an sgRNA (targeting CCAAAATGCAGCTCCGTGGG) and an ssODN template (GAGCTGTAATAAGCCCTTTCGGATGCTTGTGGCTCAGGACACTCGCCCCACGGAGCTGCATTTTGGGGAGAAATGGTTCCACA) using CRISPR/Cas9 technology. The mutation models a human SNP that was identified in a whole exome chip of rare SNPs associated with Alzheimer's disease and has been associated with protection from Alzheimer's disease. (J:101977, J:308279)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Plcg2 Mutation:  50 strains or lines available
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
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