Chst14^em6(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Chst14^em6(IMPC)Tcp
• Name: carbohydrate sulfotransferase 14; endonuclease-mediated mutation 6, The Centre for Phenogenomics
• MGI ID: MGI:6316171
• Gene: Chst14 Location: Chr2:118756978-118759066 bp, + strand Genetic Position: Chr2, 59.73 cM, cytoband E5
• Alliance: Chst14^em6(IMPC)Tcp page
• IMPC: Chst14 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele from project TCPR259 was generated at The Centre for Phenogenomics.It was targeted by CRISPR/Cas9 nickase-mediated single-strand DNA nicks to induce double-strand breaks repaired by NHEJ causing frame-shift mutation(s) in exon 1 of Chst14. The two guides were targeted to the forward strand of Chr 2:118926702:118926721 and the reverse strand of Chr 2:118926686:118926705 (GRCm38). (J:200814)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chst14 Mutation: 19 strains or lines available

References

• Original: J:200814 Toronto Centre for Phenogenomics, Strains and alleles submitted by Toronto Centre for Phenogenomics (NorCOMM2, funded by Genome Canada and Ontario Genomics Institute OGI-051). MGI Direct Data Submission. 2013
• All: 1 reference(s)