Rac1^tm1Jdo
Targeted Allele Detail

Summary

• Symbol: Rac1^tm1Jdo
• Name: Rac family small GTPase 1; targeted mutation 1, Julian Downward
• MGI ID: MGI:6314340
• Synonyms: Rac1^LSL-P29S
• Gene: Rac1 Location: Chr5:143491236-143513786 bp, - strand Genetic Position: Chr5, 82.22 cM
• Alliance: Rac1^tm1Jdo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:277539
• Parent Cell Line: PRX-B6N (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: The targeting vector is designed to insert a loxP-flanked STOP cassette, containing a neomycin resistance cassette, upstream of exon 2 of the gene. Exon 2 was modified to harbor a missense mutation at codon 29, resulting in a proline to serine mutation (P29S) commonly found in patients with cutaneous melanoma. (J:277539)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rac1 Mutation: 26 strains or lines available

References

• Original: J:277539 Lionarons DA, et al., RAC1(P29S) Induces a Mesenchymal Phenotypic Switch via Serum Response Factor to Promote Melanoma Development and Therapy Resistance. Cancer Cell. 2019 Jul 8;36(1):68-83.e9
• All: 1 reference(s)