Agrp^em1Awhi
Endonuclease-mediated Allele Detail

Summary

• Symbol: Agrp^em1Awhi
• Name: agouti related neuropeptide; endonuclease-mediated mutation 1, Anne White
• MGI ID: MGI:6304317
• Synonyms: Agrp^-
• Gene: Agrp Location: Chr8:106293327-106306477 bp, - strand Genetic Position: Chr8, 53.04 cM, cytoband D1-D2
• Alliance: Agrp^em1Awhi page

Mutation origin

• Strain of Origin: (C57BL/6J x DBA/2J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The gene was targeted using CRISPR/Cas9 technology. A mixture of four sgRNAs, two targeted upstream and two targeted downstream of the coding region of the gene, plus Cas9 mRNA was injected into (B6 x D2)F1 zygote pronuclei. Of the ten candidate mouse lines, line 5 was used for further experiments. The allele in these mice has a 912 bp deletion in the gene. (J:274740)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agrp Mutation: 20 strains or lines available

References

• Original: J:274740 Sefton C, et al., Metabolic Abnormalities of Chronic High-Dose Glucocorticoids Are Not Mediated by Hypothalamic AgRP in Male Mice. Endocrinology. 2019 May 1;160(5):964-978
• All: 1 reference(s)