Tg(Thy1-Nrg1)GIEFMhsb
Transgene Detail

Summary

• Symbol: Tg(Thy1-Nrg1)GIEFMhsb
• Name: transgene insertion GIEF, Markus H Schwab
• MGI ID: MGI:6303796
• Synonyms: HA-NRG1^GIEF
• Transgene: Tg(Thy1-Nrg1)GIEFMhsb Location: unknown
• Alliance: Tg(Thy1-Nrg1)GIEFMhsb page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-Nrg1)GIEFMhsb expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Nrg1 (MGI:96083)

• Mutation details: A modified mouse Nrg1 type IIIb1a cDNA, plus sequence for two hemagglutinin (HA) peptide tags inserted immediately after the ATG start codon, was placed in a Thy1 expression vector. This vector contains the entire Thy1 gene (including 5' promoter and 3' polyA signal sequences and neuronal-specific expression signal containing introns) with the coding region removed (from the ATG in exon 2 to the end in exon 4, including intervening introns 2 and 3). Nrg1 sequence was inserted between the remaining sequences of Thy1 exons 2 and 4. The Nrg1 modification entailed the insertion of a stop codon after the Gly-Ile-Glu-Phe (GIEF) codons 289-292 and truncation of the cDNA a short distance after. The transgene was injected into fertilized C57BL/6 oocytes. The premature stop codon results in a peptide that mimics the product that results from proteolytic cleavage of Nrg1 by Bace1. (J:262505)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:262505 Velanac V, et al., Bace1 processing of NRG1 type III produces a myelin-inducing signal but is not essential for the stimulation of myelination. Glia. 2012 Feb;60(2):203-17
• All: 3 reference(s)