Col1a1^{tm2(Gt(ROSA)26Sor-rtTA,tetO-GFP/RNAi:rluc)Hail}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm2(Gt(ROSA)26Sor-rtTA,tetO-GFP/RNAi:rluc)Hail}
• Name: collagen, type I, alpha 1; targeted mutation 2, Stephan Hailfinger
• MGI ID: MGI:6295871
• Synonyms: ROSA26-rtTA TRE-turboGFP-shRenilla
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm2(Gt(ROSA)26Sor-rtTA,tetO-GFP/RNAi:rluc)Hail} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:274052
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Reporter, Transactivator)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The transgene construct was inserted using recombinase-mediated cassette exchange (RMCE). The transgene construct contains the following elements: the Gt(ROSA)26Sor promoter driving the expression of the reverse tetracycline-controlled transactivator, and the tet operon tetracycline responsive element driving the expression of turboGFP green fluorescent protein reporter. The GFP gene is fused with sequence for a miR30-based shRNA targeting Renilla reniformis luciferase. Only upon supplying mice carrying this allele with doxycycline (or tetracycline) will the GFP/shRNA hybrid gene be expressed. (J:274052)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:274052 Schmitt A, et al., Correspondence: T cells are compromised in tetracycline transactivator transgenic mice. Cell Death Differ. 2018 Mar;25(3):634-636
• All: 1 reference(s)