Ormdl3^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ormdl3^{tm1d(EUCOMM)Wtsi}
• Name: ORM1-like 3 (S. cerevisiae); targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6287653
• Gene: Ormdl3 Location: Chr11:98472120-98478071 bp, - strand Genetic Position: Chr11, 62.07 cM
• Alliance: Ormdl3^{tm1d(EUCOMM)Wtsi} page
• IMPC: Ormdl3 gene page

Mutation origin

• Mutant Cell Line: MEPD1009_4_A10
• Germline Transmission: Earliest citation of germline transmission: J:268593
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98473017 of Chromosome 11 upstream of the critical exons 2 through 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2 through 4 at position 98475416. The critical exons 2 through 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:268593)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ormdl3 Mutation: 10 strains or lines available

References

• Original: J:268593 Zhakupova A, et al., ORMDL3 expression levels have no influence on the activity of serine palmitoyltransferase. FASEB J. 2016 Dec;30(12):4289-4300
• All: 1 reference(s)