Cyfip1^{tm2c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cyfip1^{tm2c(EUCOMM)Wtsi}
• Name: cytoplasmic FMR1 interacting protein 1; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6287442
• Synonyms: Cyfip1^fl
• Gene: Cyfip1 Location: Chr7:55491556-55582381 bp, + strand Genetic Position: Chr7, 33.42 cM, cytoband B5
• Alliance: Cyfip1^{tm2c(EUCOMM)Wtsi} page
• IMPC: Cyfip1 gene page

Mutation origin

• Mutant Cell Line: EPD0555_2_B11
• Germline Transmission: Earliest citation of germline transmission: J:272257
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 55522828 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 55525323. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:272257)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyfip1 Mutation: 74 strains or lines available

References

• Original: J:272257 Davenport EC, et al., Autism and Schizophrenia-Associated CYFIP1 Regulates the Balance of Synaptic Excitation and Inhibition. Cell Rep. 2019 Feb 19;26(8):2037-2051.e6
• All: 4 reference(s)