Gimap5^em1Khoe
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gimap5^em1Khoe
• Name: GTPase, IMAP family member 5; endonuclease-mediated mutation 1, Kasper Hoebe
• MGI ID: MGI:6286232
• Synonyms: Gimap5^flox
• Gene: Gimap5 Location: Chr6:48723131-48731134 bp, + strand Genetic Position: Chr6, 23.71 cM
• Alliance: Gimap5^em1Khoe page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: CRISPR/Cas9 was used to insert LoxP sites flanking the second exon. Because the second exon contains the translation start site, deletion of this exon results in loss of the protein. The accuracy of the insert was confirmed through sequencing. The presence of the LoxP sites was confirmed through PCR genotyping using a 5 LoxP site PCR forward, 5-GATC GTGCAACGACATGGG-3; 5 LoxP site PCR reverse, 5-ACGACCTTGCCCGACTAGAG-3; 3 LoxP site PCR forward, 5-CCACCCAAGGAGAGTTACCC-3; and 3 LoxP site PCR reverse, 5-GTCAAAGACAAGCTTCCACAGT-3 primers. (J:260013)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gimap5 Mutation: 13 strains or lines available

References

• Original: J:260013 Patterson AR, et al., Gimap5-dependent inactivation of GSK3beta is required for CD4(+) T cell homeostasis and prevention of immune pathology. Nat Commun. 2018 Jan 30;9(1):430
• All: 2 reference(s)