Gsdmd^em2Broz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gsdmd^em2Broz
• Name: gasdermin D; endonuclease-mediated mutation 2, Petr Broz
• MGI ID: MGI:6285140
• Synonyms: GSDMD^D88A
• Gene: Gsdmd Location: Chr15:75734176-75739257 bp, + strand Genetic Position: Chr15, 35.0 cM, cytoband D3-E1
• Alliance: Gsdmd^em2Broz page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR-targeting altered the sequence from AT to CC within exon 2 in order to introduce an alanine for aspartic acid at position 88 (D88A) amino acid substitution. A silent G to C mutation was also introduced to destroy the PAM sequence. (J:272519)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gsdmd Mutation: 35 strains or lines available

References

• Original: J:272519 Chen KW, et al., Extrinsic and intrinsic apoptosis activate pannexin-1 to drive NLRP3 inflammasome assembly. EMBO J. 2019 Mar 22
• All: 4 reference(s)