Mir574^tm1.1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir574^tm1.1Wtsi
• Name: microRNA 574; targeted mutation 1.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6284311
• Synonyms: Mir574^{tm1.1(mirKO)Wtsi}
• Gene: Mir574 Location: Chr5:65127661-65127738 bp, + strand Genetic Position: Chr5, 33.55 cM
• Alliance: Mir574^tm1.1Wtsi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector containing (from 5' to 3') a loxP-F3-puromycin Δtk-loxP-FRT was used to disrupt Mir574 on chromosome 5. Mir574 is located in intron 1 of the host gene Fam114a1. Cre-mediated recombination removed the floxed purumycin cassette. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mir574 Mutation: 3 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 3 reference(s)